General Information

  • ID:  hor004735
  • Uniprot ID:  A8MRK3
  • Protein name:  Protein RALF-like 35
  • Gene name:  At1g60913
  • Organism:  Arabidopsis thaliana (Mouse-ear cress)
  • Family:  Plant rapid alkalinization factor (RALF) family
  • Source:  Plant
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Arabidopsis (genus), Camelineae (tribe), Brassicaceae (family), Brassicales (order), malvids, rosids, Pentapetalae, Gunneridae, eudicotyledons, Mesangiospermae, Magnoliopsida (class), Spermatophyta, Euphyllophyta, Tracheophyta, Embryophyta, Streptophytina (subphylum), Streptophyta (phylum), Viridiplantae (kingdom), Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0019722 calcium-mediated signaling
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  RYIKYRAIAKDRVPDCTQDPKNCVRVPVNQYHLPPGCQNTTHCYREKYHI
  • Length:  50
  • Propeptide:  MAAHKMSLTSLFFVSIVIVLSLFSGFGEGRYIKYRAIAKDRVPDCTQDPKNCVRVPVNQYHLPPGCQNTTHCYREKYHI
  • Signal peptide:  MAAHKMSLTSLFFVSIVIVLSLFSGFGEG
  • Modification:  NA
  • Glycosylation:  T39 N-linked (GlcNAc...) asparagine
  • Mutagenesis:  NA

Activity

  • Function:  Cell signaling peptide that may regulate plant stress, growth, and development. Mediates a rapid alkalinization of extracellular space by mediating a transient increase in the cytoplasmic Ca(2+) concentration leading to a calcium-dependent signaling events through a cell surface receptor and a concomitant activation of some intracellular mitogen-activated protein kinases.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  16-23; 37-43
  • Structure ID:  AF-A8MRK3-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004735_AF2.pdbhor004735_ESM.pdb

Physical Information

Mass: 686406 Formula: C262H409N81O73S4
Absent amino acids: FMSW Common amino acids: PRYCKV
pI: 9.27 Basic residues: 12
Polar residues: 16 Hydrophobic residues: 10
Hydrophobicity: -104 Boman Index: -14318
Half-Life: 1 hour Half-Life Yeast: 2 min
Half-Life E.Coli: 2 min Aliphatic Index 58.4
Instability Index: 3035.6 Extinction Coefficient cystines: 7700
Absorbance 280nm: 157.14

Literature

  • PubMed ID:  NA
  • Title:  NA